Fundamental principles of next-generation sequencing

Speaker: Dr. Joshua B. Gross

Workshop Date: October 15th

Workshop Schedule

8:00am to 11:30am

  • Workshop Introduction
  • Performing a de novo versus a template assembly
  • Mapping reads from an RNA-seq project

12:00pm to 2:00pm – Lunch

2:00pm to 5:00pm

  • Generating and analyzing a SNP Report
  • Annotating a transcriptome using Blast2GO
  • The fundamentals of DNA and protein sequence analysis

Tentative Workshop Components

  • A Guide to Free Resources: for performing and analyzing next-generation sequencing datasets.
  • Introduction
  • Applications and Uses: of next-generation sequencing technology. For example a) RNA-sequencing, b) phylogenetic studies, c) GWAS.
  • Scientific talk: An example of the use of next-generation sequencing as illustrated with a published research paper (Gross et al., 2013)
  • Glossary: terms to know for NGS studies.

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